Direct location of individual sulfur atoms from weak medium-resolution SAD data
نویسندگان
چکیده
منابع مشابه
Counting individual sulfur atoms in a protein by ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry: experimental resolution of isotopic fine structure in proteins.
A typical molecular ion mass spectrum consists of a sum of signals from species of various possible isotopic compositions. Only the monoisotopic peak (e.g., all carbons are 12C; all nitrogens are 14N, etc.) has a unique elemental composition. Every other isotope peak at approximately integer multiples of approximately 1 Da higher in nominal mass represents a sum of contributions from isotope co...
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Mixtures of mineral aggregate and modified sulfur, wherein the modified sulfur functions as a binder, are termed sulfur concretes or sulfur mortars depending on the form of the mineral aggregate. Molten sulfur reacted with olefinic additive, at 130-140 ºC, and then mixed with preheated filler and aggregates. Sulfur mortar samples were prepared by mixing 32 % molten sulfur, 10 % filler and 5...
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High-quality high-multiplicity X-ray diffraction data were collected on five different crystals of thaumatin using a homogeneous-profile X-ray beam at E = 8 keV to investigate the counteracting effects of increased multiplicity and increased radiation damage on the quality of anomalous diffraction data collected on macromolecular crystals. By comparing sulfur substructures obtained from subsets...
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The location of heavy atoms belonging to the native form of a protein is a well-known technique (Rossmann, 1961; Strahs & Kraut, 1968) and relies on the combined use of normal electron density maps and maps based on the anomalous differences. While the normal map is made with mean structure amplitudes phased either by the techniques of multiple isomorphous replacement or molecular replacement, ...
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Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur S...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2004
ISSN: 0108-7673
DOI: 10.1107/s0108767304099714